Brief introduction of efficacy classification: tonic medicine efficacy: tonic liver and kidney; clear deficiency fever; genus of eye genus: Oleaceae Latin name: Fructus Ligustri Lucidi Pharmacology (toxicology): Ligustrum lucidum is very toxic to animals, rabbits take once75g of fresh ripe fruit without poisoning.
Indications: Dizziness; soft waist and knees; nocturnal emission; tinnitus; premature whiteness and hot flashes of bone steam; dark and unclear ecological environment: born in sparse forests or dense forests below 2900m above sea level, mostly cultivated in courtyards or roadsides.
Harvesting and Storage: 4 after Ligustrum Transplantation?
The results begin in 5 years. When the fruit turns black and has white powder every December, it is layed down to remove stalks, leaves and impurities. It is dried or dried in hot water.
Resource distribution: distributed in Shaanxi, Gansu, Zhejiang, Jiangsu, Yunnan, Guizhou, Guangdong and other places.
Medication contraindications: spleen and stomach deficiency cold diarrhea and yang deficiency should not be taken.
“Ben Cao Jing Shu”: when mixed with spleen and stomach medicine and pepper red warming, the same application, or you may have abdominal pain as a leak.
Medicinal properties: This product is a virgin virgin plant of the family Oleaceae.
Dry ripe fruits.
Winter fruits are harvested when they are mature, the branches and leaves are removed, and they are slightly steamed or boiled in boiling water and dried; or dried directly.
Oval, oval or kidney-shaped, 6?
5mm, diameter 3.
The surface is black-purple or gray-black, with uneven crests, stalk marks at the base or with a clipboard and short stems.
The outer skin is thin, the middle skin is soft, easy to peel off, the inner skin is woody, yellow-brown, with longitudinal ribs, and the seeds are usually 1 grain, kidney-shaped, purple-black, oily after opening.
Odorless, sweet and slightly bitter.
Medicinal properties: sweetness, bitterness, coldness, and classics: liver, kidney, efficacy: nourishing liver, kidney and yin, black eyesight.
Indications: For liver and kidney yin deficiency, backache, tinnitus, early whitening; dim eyes, dim vision; yin deficiency and fever.
Dosage: Jianbi, 10?
Lipid-lowering and anti-arteriosclerotic powder of Ligustrum lucidum 20g / only mixed into the food and fed, it can reduce the serum cholesterol and triglyceride content in experimental hyperlipidemia rabbits, thereby causing aortic lipid plaque and coronaryReduced atherosclerotic plaque formation.
Ligustrum lucidum oleanolic acid 30mg / kg was mixed into a drink and fed to rats, 0.
4% oleanolate suspension 0.
5ml was administered to rabbits by gavage. For hyperlipidemia rats, both rabbits had hypolipidemic effect.
Oleanolic acid at 30 and 60 mg / kg was fed to Japanese quail for 8 weeks, which significantly reduced serum total cholesterol, lipid peroxide, total cholesterol content in the arterial wall, and reduced the incidence of atherosclerosis.
Hypoglycemic oleanolic acid is injected subcutaneously at 50, 100 mg / kg for 7 consecutive days, which can lower blood sugar in normal mice. It has preventive and therapeutic effects on diabetes in mice caused by tetratoluenesulfonic acid, and it can also fight against epinephrine or glucose.Mice have elevated blood sugar.
Anti-liver damage oleanolic acid 30, 50, 100 mg / kg subcutaneous injection can inhibit the increase of serum alanine aminotransferase (SGPT) in rats induced by carbon tetrachloride. For rats called carbon tetrachloride, QiSubcutaneous injection of oleic acid at 50 and 100 mg / kg can also decrease SGPT.
70mg / kg oleanolic acid can reduce liver damage caused by carbon tetrachloride. Histological observation, hepatocyte vacuole degeneration, looseness, hepatocyte necrosis, and lobular interstitial toxicity are all milder than corresponding ones.
Oleanolic acid 2mg / subcutaneous injection, for 6-9 weeks, has an effect on liver cirrhosis in rats caused by high-fat food and carbon tetrachloride.
Electron microscopy observation: In carbon tetrachloride-induced liver injury rats, oleanolic acid 20 mg / subcutaneous injection, mitochondrial swelling and endothelial reticulum vesicle transformation of transformed hepatocytes were alleviated.
Effects on the body’s immune function 4.
1 Effect on non-absolute immunity
A Ligustrum lucidum that increases the number of axial leukocytes can significantly increase the number of internal leukocytes. Its active ingredient is oleanolic acid.
Alcoholic extracts can restore leukopenia in mice caused by cyclophosphamide (cy).
Steamed Ligustrum lucidum 480mg / kg has no effect on the white blood cells of normal mice, but it can correct the decrease of white blood cells caused by prednisone (25mg / kg, ip).
4.1.2 Effect on phagocytic function of reticuloendothelial system Macrophage viability was observed by clearance rate of foreign bodies in blood.
It is found that ig steamed Ligustrum lucidum 240mg / kgx5d can significantly increase the clearance index of iv carbon particles in mice and enhance the activity of the reticuloendothelial system; Ligustrum lucidum 480mg / kg has no obvious effect.
Ligustrum decoction 12.
5g / kgx7d had no significant effect on the clearance index, while 25g / kgx7d significantly inhibited the phagocytic activity of the reticuloendothelial system.
4.2.Effects on specific immunity 4.
2.1 on cellular immune function Ligustrum lucidum can significantly improve T lymphocyte function.
Ligustrum lucidum water extract 0.
02ml significantly enhanced the lymphocyte proliferation caused by its appropriate amount of PHA, COA and PWM in vitro, and also significantly enhanced the rat local graft-versus-host response (GUH) caused by xenogeneic (human) lymphocytes, possibly due to partial elimination or eliminationThe effect of weak suppressor T cells is achieved.
Ligustrum lucidum polysaccharide (LLPS) can enhance the proliferation response of mouse spleen lymphocytes to ConA stimulation: 3H-TdR incorporation value (Cpm) 10mg / kg administration group (39308).
1 ± 6208) increased by about 120% compared with the control group (17959 ± 4508), P <0.
01, 50mg / kg administration group (2979 ± 3625) increased by about 70% compared with the control group, p <0.
Ligustrum lucidum decoction can promote the delayed-type hypersensitivity induced by SRBC.
It shows that Ligustrum lucidum can enhance cellular immune function.
Oleanolic acid 40mg, taken 3 times a day for 1 to 2 months, can improve the immune function of tumor patients. The phagocytosis of macrophages and rosettes of blood lymphocytes were significantly improved by the vesicle method.
Ligustrum lucidum induces M in both water and alcohol extracts?
The effect of IL-1, and the water extract is stronger than the alcohol extract. This result reveals that the immune promotion effect of Ligustrum lucidum may be related to the induction of M?
At the same time, Ligustrum lucidum promotes the activity of T cells by enhancing the activity of cell surface receptors. Ligustrum lucidum may be a better immunoregulatory drug for the low immune function caused by too high Ts activity.
4.2.2 on humoral immune function Ligustrum lucidum has the effect of enhancing humoral immune function.
Ligustrum decoction ig12 for 7 consecutive days.
5 ～ 25g / kg can increase the weight of thymus and spleen of mouse immune organs; large dose can increase the weight of spleen of adult rats.
Steamed Ligustrum lucidum 240mg / kg, 480mg / kg × 7 days can significantly increase the spleen, thymus, abdominal lymph nodes and adrenal glands of C57BL mice.
Ligustrum lucidum polysaccharides were injected once a day for a total of 100 mg / kg for 7 days, which increased the spleen weight of LACA mice by about 40% compared with the control group, and 50 mg / kg increased the spleen weight of mice by about 30% compared with the control group.
Ligustrum lucidum decoction can significantly increase the activity of serum hemolysin antibodies in mice, and can also increase the serum IgG content of normal mice, and counteract the immunosuppressive effect of Cy;It can also increase the serum IgG in mice, and can increase the IgG content of prednisone immunosuppressed mice. It can still clearly indicate that mice remember the activity of hemoglobin antibody; Ligustrum lucidum polysaccharides against Cy50mg / kgip caused byThe tumor cell-bearing mice (S180) had a low inhibitory effect on the lymphocyte proliferation response, and Ligustrum lucidum polysaccharides obviously counteracted the immunosuppressive effect of Cy.
4.Inhibition of allergic reactions by Ligustrum lucidum 12.5,25g / kgig mice for 7d; the same dose of 5dig rats, different doses of Ligustrum lucidum on mice or rats passive skin allergic reaction (PCA) significantly inhibited.
Ligustrum lucidum decoction can reduce the percentage of degranulation of rat mast cells and prevent the increase of rat capillary permeability caused by histamine; administration before antigen challenge can inhibit DNCB-induced contact dermatitis in mice; Ligustrum lucidumThe administration of 25g / kg of seed after antigen challenge can also significantly inhibit the contact skin process of mice caused by DNCB; reduce the active and reverse passive Arthus response in rats; 20g / kg of Ligustrum lucidum significantly reduces the total serum complement of guinea pigs.
This shows that Ligustrum lucidum has obvious inhibitory effects on type Ⅰ, Ⅱ, and Ⅳ allergies.
5.The anti-inflammatory effect was confirmed by Ligustrum lucidum using various experimental antibacterial models.
5g / kg, 25g / kg daily ig, for 5 consecutive days, p-xylene caused swelling of the auricles of mice, increased capillary permeability of the abdominal cavity of mice caused by acetic acid, and carrageenan, egg white, and formaldehyde-induced rat feetThe swelling and expansion of the pad significantly inhibited; Ligustrum lucidum 20g / kg × 3 days ig can significantly reduce the release of PGE in rat tissues; Ligustrum lucidum 20g / kg × 7 days can inhibit the proliferation of rat cotton ball granulation tissue,This is accompanied by an increase in adrenal weight.
Its anti-inflammatory mechanism may involve the following aspects: ① activating the pituitary-adrenocortical system and promoting the release of corticosteroids; ② inhibiting the synthesis or release of PGE.
In addition, Ligustrum lucidum can reduce serum complement activity in guinea pigs and increase the permeability of rat skin capillaries caused by the antibiotic medium histamine. Therefore, the anti-inflammatory mechanism of Ligustrum lucidum may also include the aforementioned effects.
Anti-cancer, anti-mutant oleanolic acid has antitumor effect on mouse sarcoma-180.
Ligustrum decoction 12.
5, 25 g / kg gavage, oleanolic acid 50, 100 mg / kg subcutaneous injection, can significantly inhibit the increase of micronucleus rate in mice caused by cyclophosphamide and urethane.
Studies on the effect on the endocrine system show that Ligustrum lucidum contains both estrogen-like substances and androgen-like substances. Ligustrum lucidum 428 is contained in Ligustrum lucidum by radioimmunoassay.
31 pg / g, estradiol 139.
02pg / gram.
It is proved that Ligustrum lucidum has both insulin pill-like and estradiol-like hormone analogs, that is, the same drug has a two-way regulating effect.
Traditional Chinese medicines such as Ligustrum lucidum and kidney-yin produced estrogen-like effects on the vaginal mucosa of indifferent mice, and the number of large follicles in the ovaries of rabbits in the medication group increased significantly, and estrogen increased.
8.Impact on the hematopoietic system Ligustrum lucidum has a promoting effect on red blood cells.
Ligustrum lucidum can promote the growth of CFU-E using the diffusion box plasma clot method.
CFU-E / 5 × l4 is 128.
6 ± 11.
4 (8), CFU-E / femur was 62040 ± 5450 (8), CFU-E in femur was significantly higher than that in NS group, but CFU-D was significantly reduced.
Morphological analysis of bone marrow cells in mice after treatment showed that the percentage of red blood cells in the Ligustrum lucidum group was 47.
3 ± 2.
99 (6) is better than NS37.
3 ± 2.
The 86 (6) group increased, and the percentage of granulocytes was 37.
6 ± 3.
96 (6) was 46 compared with NS group.
6 ± 6.
26 (6) decreases, and the ratio of grain red changes accordingly.
Protective effect on chromosomal damage caused by cyclophosphamide Protective effect on chromosomal damage caused by cyclophosphamide and urethane Micronucleus test method proves that Ligustrum lucidum decoction pol2.
5 g / kg 25.
0 g / (kg
d) × 6, oleanolic acid ih50mg / kg, 100mg / (kg · d) × 7 pairs of cyclophosphamide ip50mg / (kg · d) × 2, urethane 500mg / (kg · d) × 4, caused by ipChromosome damage in mice has a protective effect and can reduce its micronucleus rate.
Anti-HpD photochlorination Ligustrum lucidum can counteract the photooxidation of HpD, and its application in vivo can significantly alleviate the photosensitivity of HpD to the skin of mice.
Ligustrum lucidum 60mg crude drug / ml can significantly reduce the increase of malondialdehyde content in red blood cells caused by HpD5ug / ml combined with light exposure for 10min, and inhibit 57.
7%, obviously against the inhibition of erythrocyte membrane acetylcholinesterase activity, resistance against 49.
O%, 120mg / ml, against supplement 53.
3%, mouse ipHpD20mg / kg, light exposure for 4h, Ligustrum lucidum 20g crude drug / kg, ip 1 time, significantly alleviate ear photosensitivity.
Ligustrum lucidum produces 1O2 less efficiently than digits.
5 × 10-4 times.
Other effects Ligustrum lucidum still has cardiovascular effects such as strengthening heart, dilating coronary vessels, dilating blood vessels; diuretic, antitussive, laxative, antibacterial and so on.
Oleanolic acid has some cardiotonic and diuretic effects; mannitol has a slowing effect; it also contains a large amount of glucose; these may be associated with strong effects.